IL16 anticorps

N° du produit ABIN5689034

Cet anticorps anti-IL16 est un anticorps Lapin Polyclonal détectant IL16 dans WB, ELISA et Neut. Adapté pour Humain.

Aperçu rapide pour IL16 anticorps (ABIN5689034)

Antigène: IL16 (Interleukin 16 (IL16))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL16 est non-conjugé

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Détail du produit anti-IL16 anticorps

Purification: Anti-hIL-16 specific antibody was purified by affinity chromatography employing immobilized hIL-16 matrix.

Immunogène: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-16 (human Interleukin-16).

Information d'application

Indications d'application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-16 (4.20 ng/mL), a concentration of 0.12 - 0.15 μ,g/mL of this antibody is required.

ELISA:

To detect hIL-16 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-16.

Sandwich:

To detect hIL-16 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-16 (XP-5170Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-16.

Western Blot:

To detect hIL-16 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-16 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Stock: -20 °C

Stockage commentaire: IL-16 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Détails sur IL16

Antigène: IL16 (Interleukin 16 (IL16))

Autre désignation: IL-16

Sujet: IL16 is a proinflammatory cytokine that is chemotactic for CD4+ T lymphocytes, monocytes, and eosinophils. IL16 is synthesized as a precursor molecule (pro IL16) of approximately 68 kDa and 631 amino acid residues lacking a signal peptide. The sequence and structure of IL16 is conserved across species. In addition to inducing chemotaxis, IL16 upregulates the IL2 receptor and upregulates HLADR expression. It also inhibits T cell receptor (TCR)/CD3 dependent activation, and suppresses HIV1 replication in vitro. Sources of IL16 include epithelial cells, mast cells, T lymphocytes (CD4+ and CD8+), macrophages, synovial fibroblasts, and eosinophils.

ID gène: 3603

UniProt: Q9UME6